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RaPID Assay PCB

Home » RaPID Assay PCB

RaPID Assay PCB Test Kit allows reliable determination of PCB and related compounds

The RaPID Assay PCB Test Kit can be used as a quantitative, semi-quantitative or qualitative enzyme immunoassay (EIA) for the analysis of PCB (polychlorinated biphenyl) in water (groundwater, surface water, well water). For applications in other matrices please contact our Technical Service department or refer to the soil application procedure provided. The RaPID Assay PCB Test Kit allows reliable and rapid screening for PCB (measured and reported as Aroclor 1254), with quantitation between 0.5 and 10 ppb (as Aroclor 1254), in water. The minimum detection level of the kit is 0.2 ppb (as Aroclor 1254.)

Downloads

• RaPID PCB Spec Sheet (150kb) download
• RaPID PCB User Guide (375kb) download

Product Description

The PCB RaPID Assay kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of PCB and related compounds. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles with antibodies specific to PCB attached. Both PCB (which may be in the sample) and the enzyme labeled PCB (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of an incubation period, a magnetic field is applied to hold the paramagnetic particles (with PCB and labeled PCB analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution.

The presence of PCB is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'- tetramethylbenzidine). The enzyme labeled PCB analog bound to the PCB antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of acid. Since the labeled PCB (conjugate) was in competition with the unlabeled PCB (sample) for the antibody sites, the color developed is inversely proportional to the concentration of PCB in the sample.

Assay Range
- Soil: 0.5 to 10.0 ppm Total PCB's as Aroclor 1254
- Water: 0.5 to 10.0 ppb Total PCB's as Aroclor 1254
- Wipes: 5 to 100 ug per wipe Total PCB's as Aroclor 1254
- Range can be extended with additional dilutions

Sampling Time
- Soil extraction time is typically 2 minutes per sample plus assay run time of approximately 60 minutes.

Required Test Materials SDI Part #
A00133 PCB Assay Test Kit: 30 Tube
A00134 PCB Assay Test Kit: 100 Tube
A00137EA SDI Sample Extraction Kit or
A00137EB ( for soil samples only )
A00137WA SDI Sample Extraction Kit or
A00137WB SDI Sample Extraction Kit ( for wipe samples only )
A00136 PCB Sample Diluent: 100 ml ( as needed )
A00235 Universal Range Extension Kit ( as needed to extend range )

Required Test Equipment
6050100 RaPID Assay Accessory Kit which contains:
A00003 RPA-I RaPID Analyzer
A00004 Magnetic Separation Rack
A00008 Repeator Pipet
A00176 Adjustable Volume Pipet
A00014 Vortex Mixer
A00131 Portable Balance
A00015 Digital Timer
A00009 Repeator Pipet Tips
A00013 Adjustable Pipet Tips